Boster Bio Anti-SMARCAD1 Antibody Picoband catalog A06049-1. Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1
Application Dilute:
Western blot, 0.1-0.5µg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml Immunocytochemistry/Immunofluorescence, 2µg/ml Immunoprecipitation, 0.5-2 µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x106 cells ELISA, 0.1-0.5µg/ml
IHC analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1).SMARCAD1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SMARCAD1 Antibody (A06049-1) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1).SMARCAD1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SMARCAD1 Antibody (A06049-1) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1).SMARCAD1 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SMARCAD1 Antibody (A06049-1) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1).SMARCAD1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-SMARCAD1 Antibody (A06049-1) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
Western blot analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates&44, Lane 2: human HL-60 whole cell lysates&44, Lane 3: human U2OS whole cell lysates&44, Lane 4: human U-87MG whole cell lysates&44, Lane 5: human HepG2 whole cell lysates&44, Lane 6: human THP-1 whole cell lysates&44, Lane 7: human A549 whole cell lysates. After Electrophoresis&44, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCAD1 antigen affinity purified polyclonal antibody (Catalog A06049-1) at 0.5 &mu,g/mL overnight at 4°,C&44, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for SMARCAD1 at approximately 150KD. The expected band size for SMARCAD1 is at 117KD.
Western blot analysis of SMARCAD1 using ant
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