E.coli-derived human ApoER2/LRP8 recombinant protein (Position: R444-D960).
Alternative Names:
LDL receptor related protein 8, APOER2, HSZ75190, LRP-8, MCI1, LRP8
Boster Bio Anti-ApoER2/LRP8 Antibody Picoband catalog A03444-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Low-density lipoprotein receptor-related protein 8
Application Dilute:
Western blot, 0.1-0.25µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 4µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human ELISA, 0.1-0.5µg/ml, -
IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2). ApoER2/LRP8 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ApoER2/LRP8 Antibody (A03444-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2). ApoER2/LRP8 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ApoER2/LRP8 Antibody (A03444-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2). ApoER2/LRP8 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ApoER2/LRP8 Antibody (A03444-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2). ApoER2/LRP8 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ApoER2/LRP8 A
Western blot analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U937 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: rat thymus tissue lysates, Lane 10: rat spleen tissue lysates, Lane 11: rat heart tissue lysates, Lane 12: mouse testis tissue lysates, Lane 13: mouse thymus tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ApoER2/LRP8 antigen affinity purified polyclonal antibody (Catalog A03444-2) at 0.25 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for ApoER2/LRP8 at approximately 106KD. The expected band size for ApoER2/LRP8 is at 106KD.
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