TJP1 Antibody - middle region

Catalog Number: ASB-ARP36636_P050
Article Name: TJP1 Antibody - middle region
Biozol Catalog Number: ASB-ARP36636_P050
Supplier Catalog Number: ARP36636_P050
Alternative Catalog Number: ASB-ARP36636_P050-25UL,ASB-ARP36636_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: IHC, WB
Species Reactivity: Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TJP1
Alternative Names: ZO-1
TJP1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. TJP1 may be involved in signal transduction at cell-cell junctions.This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 187kDa
NCBI: 7082
UniProt: Q07157
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Testis
WB Suggested Anti-TJP1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
TJP1 antibody - middle region (ARP36636_P050) in Human HepG2 using Western Blot