RAD54B Antibody - N-terminal region

Catalog Number: ASB-ARP36407_P050
Article Name: RAD54B Antibody - N-terminal region
Biozol Catalog Number: ASB-ARP36407_P050
Supplier Catalog Number: ARP36407_P050
Alternative Catalog Number: ASB-ARP36407_P050-25UL,ASB-ARP36407_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: IHC, WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RAD54B
Alternative Names: RDH54
RAD54B belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 103 kDa
NCBI: 25788
UniProt: Q9Y620
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Rabbit Anti-RAD54B Antibody
Catalog Number: ARP36407_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver
Observed Staining: Nuclear in hepatocytes, very strong signal, wide tissue distribution
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 a€'' 2.0 sec
Protocol located in Reviews and Data.
WB Suggested Anti-RAD54B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human brain
25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The peptide sequence is present in isoforms at ~97-94 kDa.
RAD54B antibody - N-terminal region (ARP36407_P050) in Human Brain using Western Blot