ID4 Antibody - middle region

Catalog Number: ASB-ARP32327_P050
Article Name: ID4 Antibody - middle region
Biozol Catalog Number: ASB-ARP32327_P050
Supplier Catalog Number: ARP32327_P050
Alternative Catalog Number: ASB-ARP32327_P050-25UL,ASB-ARP32327_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ID4
Alternative Names: IDB4, bHLHb27
Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins.Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 16kDa
NCBI: 3400
UniProt: P47928
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
WB Suggested Anti-ID4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 293T cell lysateID4 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
Lane 1: 10ug MDA-MB231 lysateLane 2: 10ug MCF7 lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
ID4
Submitted by:
Maria Teresita Branham. Facultad de Cs MAfAdicas-UNCuyo
Host: Rabbit
Target: ID4
Positive control (+): 293T Cell Lysate (2T)
Negative control (-): Human Placenta (PL)
Antibody concentration: 1ug/ml
ID4 antibody - middle region (ARP32327_P050) in Human HEK293T using Western Blot