SMAD5 Antibody - middle region

Catalog Number: ASB-ARP32006_P050
Article Name: SMAD5 Antibody - middle region
Biozol Catalog Number: ASB-ARP32006_P050
Supplier Catalog Number: ARP32006_P050
Alternative Catalog Number: ASB-ARP32006_P050-25UL,ASB-ARP32006_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SMAD5
Alternative Names: DWFC, JV5-1, MADH5
SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 51kDa
NCBI: 4090
UniProt: Q99717
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
WB Suggested Anti-SMAD5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate
Lanes:
Lane 1: 5ug of transfected 293T lysate (SMAD1)
Lane 1: 5ug of transfected 293T lysate (SMAD2)
Lane 1: 5ug of transfected 293T lysate (SMAD3)
Lane 1: 5ug of transfected 293T lysate (SMAD4)
Lane 1: 5ug of transfected 293T lysate (SMAD5)
Lane 1: 5ug of transfected 293T lysate (SMAD6)
Lane 1: 5ug of transfected 293T lysate (SMAD7)
Lane 1: 5ug of transfected 293T lysate (SMAD8)
Lane 1: 5ug of transfected 293T lysate (GFP)
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-Rabbit IgG HRP Conjugated
Secondary Antibody Dilution:
1:10,000
Gene Name:
SMAD5
Submitted by:
Amanda Urick, Medical College of Wisconsin
Host: Rat
Target Name: SMAD5
Sample Tissue: Rat Liver
Antibody Dilution: 1ug/ml
SMAD5 antibody - middle region (ARP32006_P050) in Human HepG2 using Western Blot