E2F1 Antibody - middle region

Catalog Number: ASB-ARP31171_P050
Article Name: E2F1 Antibody - middle region
Biozol Catalog Number: ASB-ARP31171_P050
Supplier Catalog Number: ARP31171_P050
Alternative Catalog Number: ASB-ARP31171_P050-25UL,ASB-ARP31171_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: WB
Species Reactivity: Human
Alternative Names: RBP3, E2F-1, RBAP1, RBBP3
The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 47kDa
NCBI: 1869
UniProt: Q01094
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Rabbit Anti-E2F1 Antibody
Catalog Number: ARP31171_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
WB Suggested Anti-E2F1 Antibody
Titration: 1.0 ug/ml
Positive Control: Fetal Lung
Lanes:
1. 30 ug Cytosol extract: Untransfected cells 2. 30 ug Cytosol extract: Cells transfected with control (non-targeting) siRNA 3. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNA 4. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNA
Primary Antibody Dilution:
1:2000
Secondary Antibody:
Goat anti-rabbit HRP
Secondary Antibody Dilution:
1:2000
Gene Name:
E2F1
Submitted by:
Ewelina Swiderek, Insitute of Immunology and Experimental Therapy, Wroclaw, Poland
E2F1 antibody - middle region (ARP31171_P050) in Human Fetal Lung using Western Blot