Western blot analysis: 0.5-2 µg/ml, FlowCytometry: 0.5-1 µg/10 6 Cells
Figure-1: Western blot analysis of DFF-40. Anti-DFF-40 antibody (Clone: ABM1H15) was used at 1 µg/ml on (1) h Spleen, (2) h Brain and (3) Ramos lysates.
Figure-2: Western blot analysis of DFF-40. Anti-DFF-40 antibody (Clone: ABM1H15) was used at 0.5 µg/ml on (1) EL-4, (2) 3T3 and (3) RAW lysates.
Figure-3: Intracellular flowcytometry analysis of DFF-40 on HeLa cells using 0.5 µg/10 6 Cells of Anti-DFF-40 antibody (Clone: ABM1H15). Green represent isotype control and red represent Anti DFF-40 antibody (Abeomics 10-6008). Goat anti mouse PE conjugated was used as the secondary antibody.
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