Rat NKp46 is an activating receptor expressed on all NK cells in all rat strains tested. NKp46 is the defining surface marker for rat NK cells. No expression of NKp46 has so far been reported on non-NK cells in the rat. NKp46 associates with the activating adaptor proteins FcepsilonRIgamma and CD3zeta and activates NK cell cytotoxicity and IFNgamma production.
Facs anaysis: 1-2 µg/10 6 Cells, Western blot analysis: 1-2 µg/ml, Immunoprecipitation: 1-2 µg/ml Reference: Ingunn H. Westgaard, Siri F. Berg, John T. Vaage, Lawrence L. Wang, Wayne M. Yokoyama, Erik Dissen, and Sigbjrn Fossum , Rat NKp46 activates natur
Figure-2: Flow cytometry analysis of anti-NKp46 (Cat.No.: 10-4194) in IL-2-stimulated Rat NK cells. Black line represent anti-NKp46 (Clone:WEN23), dotted line represent Secondary antibody control.
Figure-1: Cell surface staining of rat splenocytes. Green represents isotype control, red represents anti-rat NKP46 (10-4194) antibody. 0.2 µg antibody was used. Goat anti-mouse FITC was used as secondary antibody.
Figure 4: Immuno precipitation of anti-NKp46 from surface biotinylated, transiently transfected 293T cells using HRP-conjugated streptavidin under Non-reducing (NR) and reducing (R) conditions.
Figure-3: Western blot analysis of anti-NKp46 in 293T cells transfected with a rat NKp46 expression construct and empty vector (EV) lysates at Non-reducing (NR) and reducing (R) condition.
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