Recombinant Human Wnt-3a Surrogate Protein
Catalog Number:
ABB-RP01618SLQ
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| Article Name: | Recombinant Human Wnt-3a Surrogate Protein |
| Biozol Catalog Number: | ABB-RP01618SLQ |
| Supplier Catalog Number: | RP01618SLQ |
| Alternative Catalog Number: | ABB-RP01618SLQ-100UG,ABB-RP01618SLQ-10UG,ABB-RP01618SLQ-20UG,ABB-RP01618SLQ-50UG |
| Manufacturer: | ABclonal |
| Host: | Human |
| Category: | Proteine/Peptide |
| Species Reactivity: | Human |
| Alternative Names: | WNT3A,Protein Wnt-3a,Wnt3a Surrogate |
| Recombinant Human Wnt-3a Surrogate Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence of Human Wnt-3a Surrogate fused with hFC at the C-terminus. |
| Concentration: | < 0.01 EU/µg of the protein by LAL method |
| Molecular Weight: | 67.52 kDa |
| Tag: | C-hFc |
| Source: | HEK293 Cells |
| Purity: | 95 % as determined by SDS-PAGE, 95% as determined by HPLC. |
| Form: | Lyophilized from sterile 0.22 µm filtered solution in PBS buffer with Trehalose, Glycerol, and Tween80, pH 7.4. |
| Target: | Wnt Surrogate Protein |
| Application Dilute: | Lyophilized from sterile 0.22 µm filtered solution in PBS buffer with Trehalose, Glycerol, and Tween80, pH 7.4. |
| Application Notes: | ResearchArea: Cytokines & Cytokine receptors. Shipping: Dry Ice |
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Recombinant Human Wnt-3a Surrogate Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
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Recombinant Human Wnt-3a Surrogate Protein is greater than 95% as determined by SEC-HPLC. |
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Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50for this effect is 7.38-29.54 ng/mL ,corresponding to a specific activity of 3.39*10<,sup>,4<,/sup>,~1.35*10<,sup>,5<,/sup>, units/mg. |
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Human stomach organoids organoids were cultured with EGF(Cat. RP03287), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ). |
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Mouse large intestinal organoids were cultured with EGF(Cat.RP03287),NOG(Cat. RP01237), RSPO1(Cat. RP00071),WNT-3a(Cat.RP01618SLQ) |
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Human liver organoids were cultured with EGF(Cat. RP03287), HGF(Cat. RP01602), FGF2(Cat. RP01042), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ). |
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Human kidney organoids were cultured with EGF(Cat. RP03287), FGF2(Cat. RP01042), FGF7(Cat. RP01717), FGF9(Cat. RP01710), FGF10(Cat. RP01140), IGF-(Cat. RP00996), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RP01343) was used to induce the establishment of cell polarity. |
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Human gastric cancer organoid were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), FGF10(Cat. RP01140), RSPO1(Cat. RP00071), Wnt-3a Surrogate(Cat. RP01618SLQ), A83-01(Cat. RPM0008), Y-27632 dihydrochloride(Cat.RPM0007), Gastrin 1(Cat. RPM0011), AB-27 Supplement(Cat. RPM0047LQ), N-2 Supplement(Cat. RPM0046LQ). |
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Mouse lung organoids were cultured in Matrigel (Cat. RPM0003) with medium supplemented with EGF (Cat. RP03287), Noggin (Cat. RP01237), RSPO1 (Cat. RP00071), Wnt-3a (Cat. RP01618SLQ), and FGF-10 (Cat. RP01140). After 8 days of culture, the expression of lineage markers, including E-Cadherin (Cat. A20798), AQP5 (Cat. A23292), and CD49F (Cat. A26707), was assessed by ultra-plex immunofluorescence staining. |
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