Phospho-RPA2-Ser33 Rabbit mAb, Unconjugated

Article Name:	Phospho-RPA2-Ser33 Rabbit mAb, Unconjugated
Biozol Catalog Number:	ABB-AP1479
Supplier Catalog Number:	AP1479
Alternative Catalog Number:	ABB-AP1479-100UL,ABB-AP1479-500UL,ABB-AP1479-20UL,ABB-AP1479-1000UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	REPA2, RPA32, RP-A p32, RP-A p34

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.

Molecular Weight:	29 kDa
NCBI:	6118
UniProt:	P15927
Purity:	Affinity purification
Sequence:	PAPSQA
Target:	RPA2

Application Dilute:	WB,1:1000 - 1:4000\|IF-P,1:50-1:200\|IHC-P,1:500 - 1:1000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair. Shipping: Ice Bag

	Immunohistochemistry analysis of paraffin-embeddedMouse testis tissue usingPhospho-RPA2-Ser33 Rabbit mAb(AP1479) at a dilution of1:800 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse brain tissue usingPhospho-RPA2-Ser33 Rabbit mAb(AP1479) at a dilution of1:800 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Western blot analysis of lysates from NIH/3T3 cells usingPhospho-RPA2-Ser33 Rabbit mAb (AP1479) at1:1000 dilution. NIH/3T3 cells were treated with Camptothecin (1 µM) at 37°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:10s.
	Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingPhospho-RPA2-Ser33 Rabbit mAb(AP1479) at a dilution of1:800 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingPhospho-RPA2-Ser33 Rabbit mAb(AP1479) at a dilution of1:800 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse kidney tissue usingPhospho-RPA2-Ser33 Rabbit mAb(AP1479) at a dilution of1:800 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Confocal imaging of paraffin-embedded Mouse testis and Mouse testis treated with lambdapp tissue using Phospho-RPA2-Ser33 Rabbit mAb (AP1479, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Mouse kidney and Mouse kidney treated with lambdapp tissue using Phospho-RPA2-Ser33 Rabbit mAb (AP1479, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.