pan Phospho-Serine/Threonine Rabbit mAb, Unconjugated
Catalog Number:
ABB-AP1475
- Images (9)
| Article Name: | pan Phospho-Serine/Threonine Rabbit mAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP1475 |
| Supplier Catalog Number: | AP1475 |
| Alternative Catalog Number: | ABB-AP1475-1000UL,ABB-AP1475-20UL,ABB-AP1475-100UL,ABB-AP1475-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC-P, IP, WB |
| Species Reactivity: | All |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Protein phosphorylation is one of the main key regulatory mechanisms by which extracellular signals are conveyed. Global alteration of signal transduction by inhibition of serine/threonine dephosphorylation has recently been shown to markedly potentiate cancer cell killing by the DNA-methylating drug, temozolomide. |
| Purity: | Affinity purification |
| Target: | pan-Phospho-S/T |
| Application Dilute: | WB,1:1000 - 1:4000|IP,2µg-4µg antibody for 200µg-400µg extracts of whole cells|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat,Other (Wide Range Predicted). ResearchArea: Protein phosphorylation,Protein phosphorylation,Protein phosphorylation. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embeddedHuman liver tissue usingpan Phospho-Serine/Threonine Rabbit mAb(AP1475) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman colon tissue usingpan Phospho-Serine/Threonine Rabbit mAb(AP1475) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Western blot analysis of lysates from A-431 cells usingpan Phospho-Serine/Threonine Rabbit mAb (AP1475) at1:1000 dilution. A-431 cells were treated with Calyculin A (200 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:90s. |
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Immunohistochemistry analysis of paraffin-embeddedHuman spleen tissue usingpan Phospho-Serine/Threonine Rabbit mAb(AP1475) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman thyroid tissue usingpan Phospho-Serine/Threonine Rabbit mAb(AP1475) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingpan Phospho-Serine/Threonine Rabbit mAb(AP1475) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedMouse testis tissue usingpan Phospho-Serine/Threonine Rabbit mAb(AP1475) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingpan Phospho-Serine/Threonine Rabbit mAb(AP1475) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunoprecipitation of pan Phospho-Serine/Threonine from 300 µg extracts of A-431 cells treated with calyculin A (200nM,30min) was performed using 2 µg of pan Phospho-Serine/Threonine Rabbit mAb (AP1475). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using pan Phospho-Serine/Threonine Rabbit mAb (AP1475) at a dilution of 1:3000. |
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