Phospho-IRE1-S724 Rabbit mAb, Unconjugated

Article Name:	Phospho-IRE1-S724 Rabbit mAb, Unconjugated
Biozol Catalog Number:	ABB-AP1442
Supplier Catalog Number:	AP1442
Alternative Catalog Number:	ABB-AP1442-500UL,ABB-AP1442-100UL,ABB-AP1442-20UL,ABB-AP1442-1000UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	IRE1, IRE1P, IRE1a, hIRE1p, Phospho-IRE1-S724

This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.

Clonality:	Monoclonal
Clone Designation:	[ARC62282]
Molecular Weight:	110kDa
NCBI:	2081
UniProt:	O75460
Purity:	Affinity purification
Sequence:	RHSFS
Target:	ERN1
Antibody Type:	Primary Antibody

Application Dilute:	WB,1:1000 - 1:5000\|IHC-P,1:500 - 1:2000\|IF/ICC,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Kinase,Cell Biology Developmental Biology,Apoptosis,Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag

	Western blot analysis of lysates from C6 cells using Phospho-IRE1-S724 Rabbit mAb (AP1442) at 1:3000 dilutionincubated overnight at 4°C. C6 cells were treated with CA (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of HeLa treated with CA and HeLa cells using Phospho-IRE1-S724 Rabbit mAb (AP1442) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Western blot analysis of lysates from HeLa cells, using Phospho-IRE1-S724 Rabbit mAb (AP1442) at 1:3000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue, untreated(left) and lambda phosphatase-treated(right), using Phospho-IRE1-S724 Rabbit mAb (AP1442) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat skin tissue, untreated(left) and lambda phosphatase-treated(right), using Phospho-IRE1-S724 Rabbit mAb (AP1442) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue, untreated(left) and lambda phosphatase-treated(right), using Phospho-IRE1-S724 Rabbit mAb (AP1442) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat brain tissue, untreated(left) and lambda phosphatase-treated(right), using Phospho-IRE1-S724 Rabbit mAb (AP1442) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue, untreated(left) and lambda phosphatase-treated(right), using Phospho-IRE1-S724 Rabbit mAb (AP1442) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue, untreated(left) and lambda phosphatase-treated(right), using Phospho-IRE1-S724 Rabbit mAb (AP1442) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.