Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb, Unconjugated
Catalog Number:
ABB-AP1430
- Images (8)
| Article Name: | Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP1430 |
| Supplier Catalog Number: | AP1430 |
| Alternative Catalog Number: | ABB-AP1430-1000UL,ABB-AP1430-500UL,ABB-AP1430-20UL,ABB-AP1430-100UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. |
| Application Dilute: | WB,1:2000 - 1:10000|IHC-P,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Protein phosphorylation. Shipping: Ice Bag |
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Western blot analysis of lysates from C2C12 cells, using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at 1:10000 dilution. C2C12 cells were treated by lambda-PP mixed solution (1ul) at 30°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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Immunohistochemistry analysis of paraffin-embeddedRat kidney tissue usingPhospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb(AP1430) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Western blot analysis of lysates from RD cells, using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at 1:10000 dilution. RD cells were treated by lambda-PP mixed solution (1ul) at 30°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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Immunohistochemistry analysis of paraffin-embeddedHuman tonsil tissue usingPhospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb(AP1430) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedMouse intestin tissue usingPhospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb(AP1430) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedRat intestine tissue usingPhospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb(AP1430) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedMouse kidney tissue usingPhospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb(AP1430) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman breast tissue usingPhospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb(AP1430) at a dilution of 1:300 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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