Phospho-SMC1-S957 Rabbit mAb, Unconjugated

Article Name:	Phospho-SMC1-S957 Rabbit mAb, Unconjugated
Biozol Catalog Number:	ABB-AP1145
Supplier Catalog Number:	AP1145
Alternative Catalog Number:	ABB-AP1145-1000UL,ABB-AP1145-20UL,ABB-AP1145-100UL,ABB-AP1145-500UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	SMC1, SMCB, CDLS2, DEE85, SB1.8, EIEE85, SMC1L1, DXS423E, SMC1alpha, Phospho-SMC1-S957

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Clonality:	Monoclonal
Clone Designation:	[ARC1606]
Molecular Weight:	143kDa
NCBI:	8243
UniProt:	Q14683
Purity:	Affinity purification
Sequence:	GSSQG
Target:	SMC1A
Antibody Type:	Primary Antibody

Application Dilute:	WB,1:500 - 1:2000\|IHC-P,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,ATM Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cell Cycle. Shipping: Ice Bag

	Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SMC1-S957 Rabbit mAb (AP1145) at 1:1000 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunohistochemistry analysis of paraffin-embeddedHuman colon carcinoma tissue usingPhospho-SMC1-S957 Rabbit mAb(AP1145) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Western blot analysis of lysates from 293T cells, using Phospho-SMC1-S957 Rabbit mAb (AP1145) at 1:1000 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry analysis of paraffin-embeddedMouse testis tissue usingPhospho-SMC1-S957 Rabbit mAb(AP1145) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse kidney tissue usingPhospho-SMC1-S957 Rabbit mAb(AP1145) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat lung tissue usingPhospho-SMC1-S957 Rabbit mAb(AP1145) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman esophagus tissue usingPhospho-SMC1-S957 Rabbit mAb(AP1145) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman small intestine tissue usingPhospho-SMC1-S957 Rabbit mAb(AP1145) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.