Phospho-IkappaBalpha-S36 Rabbit mAb, Unconjugated

Article Name:	Phospho-IkappaBalpha-S36 Rabbit mAb, Unconjugated
Biozol Catalog Number:	ABB-AP0999
Supplier Catalog Number:	AP0999
Alternative Catalog Number:	ABB-AP0999-500UL,ABB-AP0999-1000UL,ABB-AP0999-20UL,ABB-AP0999-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	IKBA, MAD-3, NFKBI, EDAID2, Phospho-IkappaBalpha-S36

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Clonality:	Monoclonal
Clone Designation:	[ARC1543]
Molecular Weight:	36 kDa
NCBI:	4792
UniProt:	P25963
Purity:	Affinity purification
Sequence:	LDSMK
Target:	NFKBIA
Antibody Type:	Primary Antibody

Application Dilute:

WB,1:1000 - 1:10000|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Application Notes:

Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,MAPK-Erk Signaling Pathway,ATM Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,NF-kB Signaling Pathway,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling. Shipping: Ice Bag

	Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Western blot analysis of various lysates using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at 1:1000 dilution. THP-1 cells were treated with PMA/TPA (80 nM) at 37°C for overnight or treated with LPS (1 µg/mL) at 37°C for 6 hours. Both NIH/3T3 cells and C6 cells were treated with TNF-alpha (20 ng/mL) at 37°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human spleen using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.