alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A7248
- Images (9)
| Article Name: | alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A7248 |
| Supplier Catalog Number: | A7248 |
| Alternative Catalog Number: | ABB-A7248-20UL,ABB-A7248-100UL,ABB-A7248-500UL,ABB-A7248-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | ACTSA, alpha-Smooth Muscle Actin (ACTA2) |
| This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. |
| Application Dilute: | WB,1:500 - 1:1000|IF-P,1:50 - 1:200|IHC-P,1:20 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Microfilaments,Actins,Death Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases Markers,Other Neurological disorders,Stem Cells,Mesenchymal Stem Cells,Cardiovascular,Heart,Contractility. Shipping: Ice Bag |
|
|
Immunohistochemistry analysis of paraffin-embedded human colon tissue using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A7248) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
|
|
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A7248) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
|
|
Western blot analysis of lysates from Mouse heart, using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A7248) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
|
|
Immunohistochemistry analysis of paraffin-embedded human esophagus tissue using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A7248) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
|
|
Immunohistochemistry analysis of paraffin-embedded human liver cancer tissue using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A7248) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse testis tissue using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A7248) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
|
|
Immunohistochemistry analysis of paraffin-embedded rat colon tissue using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A7248) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
|
|
Immunofluorescence analysis of paraffin-embedded Human colon CA using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A7248) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
|
|
Immunofluorescence analysis of paraffin-embedded mouse large intestine using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A7248) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
Product Guarantee and Expert Support
