PP1 beta Rabbit mAb, Unconjugated, Monoclonal

Article Name:	PP1 beta Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A4364
Supplier Catalog Number:	A4364
Alternative Catalog Number:	ABB-A4364-100UL,ABB-A4364-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	MP, PP1B, PP1c, NSLH2, PP-1B, PPP1CD, PP1beta, PPP1beta, HEL-S-80p, PP1 beta

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.

Clonality:	Monoclonal
Molecular Weight:	37kDa
NCBI:	5500
UniProt:	P62140
Purity:	Affinity purification
Target:	PPP1CB

Application Dilute:	WB,1:1000 - 1:6000\|IHC-P,1:500 - 1:1000\|IF/ICC,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Cancer,Signal Transduction,MAPK-Erk Signaling Pathway,Endocrine Metabolism,Lipid Metabolism,Insulin Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Dopamine Signaling in Parkinsons Disease. Shipping: Ice Bag

	Western blot analysis of various lysates using PP1 beta Rabbit mAb (A4364) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingPP1 beta Rabbit mAb(A4364) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingPP1 beta Rabbit mAb(A4364) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman breast cancer tissue usingPP1 beta Rabbit mAb(A4364) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman esophagus tissue usingPP1 beta Rabbit mAb(A4364) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman pancreas tissue usingPP1 beta Rabbit mAb(A4364) at a dilution of 1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunofluorescence analysis of NIH-3T3 cells using PP1 beta Rabbit mAb (A4364) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using PP1 beta Rabbit mAb (A4364) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.