CD44 Rabbit PolymAb, Unconjugated, Monoclonal

Article Name:	CD44 Rabbit PolymAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A28567PM
Supplier Catalog Number:	A28567PM
Alternative Catalog Number:	ABB-A28567PM-500UL,ABB-A28567PM-100UL,ABB-A28567PM-1000UL,ABB-A28567PM-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Mouse
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	Ly-24, Pgp-1, HERMES

Enables cargo receptor activity, hyaluronic acid binding activity, and type II transforming growth factor beta receptor binding activity. Contributes to cytokine binding activity and cytokine receptor activity. Involved in several processes, including hyaluronan catabolic process, negative regulation of T cell activation, and regulation of intracellular signal transduction. Acts upstream of or within several processes, including Wnt signaling pathway, morphogenesis of a branching epithelium, and wound healing involved in inflammatory response. Located in basolateral plasma membrane, external side of plasma membrane, and microvillus. Part of macrophage migration inhibitory factor receptor complex. Is expressed in several structures, including alimentary system, branchial arch, central nervous system, genitourinary system, and limb. Human ortholog(s) of this gene implicated in breast carcinoma (multiple), carcinoma (multiple), and prostate cancer. Orthologous to human CD44 (CD44 molecule (IN blood group)).

Clonality:	Monoclonal
Molecular Weight:	40-86 kDa
NCBI:	12505
UniProt:	P15379
Purity:	Affinity purification
Target:	Cd44

Application Dilute:

WB,1:3000 - 1:10000|IF/ICC,1:200 - 1:800|IHC-P,1:5000 - 1:20000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (1:10000),a sequential dilu

Application Notes:

Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Immunology, Cell Type Markers, CD, AdhesionStem ,Cells Mesenchymal ,Stem Cells ,Surface Molecules,Cancer ,Tumor immunology, CD markers,Cancer Tumor, biomarkers ,Other,Stem Cells ,Hematopoietic ,Progenitors, Lymphoid ,T Lymphocytic Lineage,Stem Cells ,Hematopoietic Progenitors, Myeloid, Dendritic Cell Lineage,StImmunology, Cell Type Markers, CD, AdhesionStem ,Cells Mesenchymal ,Stem Cells ,Surface Molecules,Cancer ,Tumor immunology, CD markers,Cancer Tumor, biomarkers ,Other,Stem Cells ,Hematopoietic ,Progenitors, Lymphoid ,T Lymphocytic Lineage,Stem Cells ,Hematopoietic Progenitors, Myeloid, Dendritic Cell Lineage,Stem Cells ,Hematopoietic Progenitors ,Myeloid ,Neutrophil Lineage,Kits/ Lysates/ Other Kits ,ELISA Kits, ELISA Kits, CD markers ELISA kits.em Cells ,Hematopoietic Progenitors ,Myeloid ,Neutrophil Lineage,Kits/ Lysates/ Other Kits ,ELISA Kits, ELISA Kits, CD markers ELISA kits. Shipping: Ice Bag

	Western blot analysis of various lysates using CD44 Rabbit PolymAb (A28567PM) at 1:5000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10 s.
	Immunohistochemistry analysis of paraffin-embedded Mouse intestine tissue using CD44 Rabbit PolymAb (A28567PM) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using CD44 Rabbit PolymAb (A28567PM) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using CD44 Rabbit PolymAb (A28567PM) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat skin tissue using CD44 Rabbit PolymAb (A28567PM) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of HeLa cells using CD44 Rabbit PolymAb (A28567PM, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of NIH/3T3 cells using CD44 Rabbit PolymAb (A28567PM, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of PC-12 cells using CD44 Rabbit PolymAb (A28567PM, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.