GAD65/GAD2 Mouse mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A27976
- Images (8)
| Article Name: | GAD65/GAD2 Mouse mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A27976 |
| Supplier Catalog Number: | A27976 |
| Alternative Catalog Number: | ABB-A27976-1000UL,ABB-A27976-100UL,ABB-A27976-20UL,ABB-A27976-500UL |
| Manufacturer: | ABclonal |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | GAD65 |
| This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. |
| Application Dilute: | WB,1:2500 - 1:10000|IF-P,1:200 - 1:800|IHC-P,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Endocrine Metabolism,Amino acid metabolism,Neuroscience. Shipping: Ice Bag |
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Western blot analysis of various lysates using GAD65/GAD2 Mouse mAb (A27976) at 1:5000 dilutionincubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): Mouse liver Exposure time: 45s. |
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Western blot analysis of lysates from Rat brain using GAD65/GAD2 Mouse mAb (A27976) at 1:5000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
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Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using GAD65/GAD2 Mouse mAb (A27976) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using GAD65/GAD2 Mouse mAb (A27976) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat pancreas tissue using GAD65/GAD2 Mouse mAb (A27976) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using GAD65/GAD2 Mouse mAb (A27976) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Confocal imaging of paraffin-embedded Mouse brain tissue using GAD65/GAD2 Mouse mAb (A27976, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
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Confocal imaging of paraffin-embedded Rat brain tissue using GAD65/GAD2 Mouse mAb (A27976, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
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