cGAS Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A27527
- Images (8)
| Article Name: | cGAS Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A27527 |
| Supplier Catalog Number: | A27527 |
| Alternative Catalog Number: | ABB-A27527-100UL,ABB-A27527-20UL,ABB-A27527-1000UL,ABB-A27527-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | MB21D1, h-cGAS, C6orf150 |
| Enables several functions, including 2,3-cyclic GMP-AMP synthase activity, chromatin binding activity, and phosphatidylinositol-4,5-bisphosphate binding activity. Involved in several processes, including cellular response to exogenous dsRNA, positive regulation of intracellular signal transduction, and regulation of defense response. Located in several cellular components, including cytosol, nucleus, and site of double-strand break. |
| Application Dilute: | WB,1:1000 - 1:2000|IHC-P,1:50 - 1:200||IF/ICC,1:100 - 1:400|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Cell Biology Developmental Biology. Shipping: Ice Bag |
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Western blot analysis of various lysates using cGAS Rabbit mAb (A27527) at 1:1000 dilutionincubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): Hep G2. Exposure time: 90 s. |
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Immunohistochemistry analysis of paraffin-embedded Human colon tissue using cGAS Rabbit mAb (A27527) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human breast tissue using cGAS Rabbit mAb (A27527) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using cGAS Rabbit mAb (A27527) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using cGAS Rabbit mAb (A27527) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using cGAS Rabbit mAb (A27527) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using cGAS Rabbit mAb (A27527) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Confocal imaging of HT-29 cells (postive) and Hep G2 cells (negative) using cGAS Rabbit mAb (A27527, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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