NFKB1 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	NFKB1 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A27463
Supplier Catalog Number:	A27463
Alternative Catalog Number:	ABB-A27463-1000UL,ABB-A27463-500UL,ABB-A27463-20UL,ABB-A27463-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ChIP, ELISA, IF, IHC-P, IP, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	KBF1, EBP-1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B1, NF-kappabeta

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.

Clonality:	Monoclonal
Molecular Weight:	105 kDa
NCBI:	4790
UniProt:	P19838
Purity:	Affinity purification
Target:	NFKB1

Application Dilute:

WB,1:1000 - 1:2000|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|IF-P,1:100 - 1:200|IHC-P,1:200 - 1:400|ELISA,Recommended starting concentration is 1 µg/mL|ChIP,5µg antibody for 5µg-20µg of Chromatin. Please optimize the concentration base

Application Notes:

Cross-Reactivity: Human,Mouse. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Jak-Stat-IL-6 Receptor Signaling Pathway,NF-kB Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway. Shipping: Ice Bag

	Western blot analysis of various lysates using NFKB1 Rabbit mAb (A27463) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10 s.
	Western blot analysis of lysates from NIH/3T3 cells using NFKB1 Rabbit mAb (A27463) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90 s.
	Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using NFKB1 Rabbit mAb (A27463) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human colon tissue using NFKB1 Rabbit mAb (A27463) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using NFKB1 Rabbit mAb (A27463) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using NFKB1 Rabbit mAb (A27463) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunoprecipitation of NFKB1 from 300 µg extracts of Raji cells was performed using 1 µg of NFKB1 Rabbit mAb (A27463). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using NFKB1 Rabbit mAb (A27463) at a dilution of 1:1000.
	Chromatin immunoprecipitation was performed with 20 µg of cross-linked chromatin from MCF7, using 3 µg of NFKB1 Rabbit mAb (A27463) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.