Fast Myosin Skeletal Heavy chain Rabbit mAb, Unconjugated, Monoclonal

Article Name:	Fast Myosin Skeletal Heavy chain Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A27146
Supplier Catalog Number:	A27146
Alternative Catalog Number:	ABB-A27146-100UL,ABB-A27146-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	MYHa, HEL71, MYHSA1, MyHC-2x, MyHC-2X/D

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.

Clonality:	Monoclonal
Molecular Weight:	223kDa
NCBI:	4619
UniProt:	P12882
Purity:	Affinity purification
Target:	MYH1

Application Dilute:	WB,1:6000 - 1:12000\|IF-P,1:200 - 1:800\|IHC-P,1:500 - 1:2000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Invasion and Metastasis,Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Motor Proteins,Extracellular Matrix,Stem Cells. Shipping: Ice Bag

	Western blot analysis of various lysates using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146) at 1:12000 dilutionincubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): Mouse brain Exposure time: 90s.
	Western blot analysis of lysates from Rat heart using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146) at 1:12000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human heart tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of paraffin-embedded Human skeletal muscle tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Mouse skeletal muscle tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Rat skeletal muscle tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.