PDGFRa Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A26747
- Images (8)
| Article Name: | PDGFRa Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A26747 |
| Supplier Catalog Number: | A26747 |
| Alternative Catalog Number: | ABB-A26747-100UL,ABB-A26747-1000UL,ABB-A26747-20UL,ABB-A26747-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, IP, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | CD140A, PDGFR2, PDGFR-2 |
| This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. |
| Application Dilute: | WB,1:500 - 1:2000|IHC-P,1:100 - 1:400|IF/ICC,1:200 - 1:1000|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Kinase,Tyrosine kinases,Cell Biology Developmental Biology,Growth factors,Immunology Inflammation,CDs,Cardiovascular,Angiogenesis. Shipping: Ice Bag |
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Western blot analysis of lysates from Mouse heart using PDGFRa Rabbit mAb (A26747) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using PDGFRa Rabbit mAb (A26747) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PDGFRa Rabbit mAb (A26747) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human colon tissue using PDGFRa Rabbit mAb (A26747) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using PDGFRa Rabbit mAb (A26747) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using PDGFRa Rabbit mAb (A26747) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using PDGFRa Rabbit mAb (A26747) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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Confocal imaging of A-204 cells using PDGFRa Rabbit mAb (A26747, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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