AFG3L2 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	AFG3L2 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A26340
Supplier Catalog Number:	A26340
Alternative Catalog Number:	ABB-A26340-100UL,ABB-A26340-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, IP, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	OPA12, SCA28, SPAX5

This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.

Clonality:	Monoclonal
Molecular Weight:	89kDa
NCBI:	10939
UniProt:	Q9Y4W6
Purity:	Affinity purification
Target:	AFG3L2

Application Dilute:	WB,1:1000 - 1:5000\|IHC-P,1:50 - 1:200\|IF/ICC,1:50 - 1:200\|IP,0.5µg-4µg antibody for 400µg-800µg extracts of whole cells\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Signal Transduction,Cell Biology Developmental Biology,Ubiquitin,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Neuroscience,Neurodegenerative Diseases,Neurodegenerative Diseases Markers,Other Neurological disorders. Shipping: Ice Bag

	Western blot analysis of various lysates using AFG3L2 Rabbit mAb (A26340) at 1:2000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using AFG3L2 Rabbit mAb (A26340) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using AFG3L2 Rabbit mAb (A26340) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using AFG3L2 Rabbit mAb (A26340) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human colon tissue using AFG3L2 Rabbit mAb (A26340) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
	Confocal immunofluorescence analysis of NIH/3T3 cells using AFG3L2 Rabbit mAb (A26340, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal immunofluorescence analysis of C6 cells using AFG3L2 Rabbit mAb (A26340, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Immunoprecipitation of AFG3L2 from 600 µg extracts of Mouse liver tissue was performed using 1 µg of AFG3L2 Rabbit mAb (A26340). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using AFG3L2 Rabbit mAb (A26340) at a dilution of 1:1000.