CD79b Rabbit mAb, Unconjugated, Monoclonal

Article Name:	CD79b Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A25358
Supplier Catalog Number:	A25358
Alternative Catalog Number:	ABB-A25358-100UL,ABB-A25358-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, FC, IF, WB
Species Reactivity:	Mouse
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	B29, Igb, Igbeta, Ig-beta

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.

Clonality:	Monoclonal
Molecular Weight:	25kDa
NCBI:	15985
UniProt:	P15530
Purity:	Affinity purification
Target:	Cd79b

Application Dilute:	WB,1:1000 - 1:4000\|IF/ICC,1:200 - 1:2000\|IF-P,1:200 - 1:2000\|FC,1:500 - 1:1000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Mouse,Rat. ResearchArea: Immunology Inflammation,CDs,B Cell Receptor Signaling Pathway. Shipping: Ice Bag

	Western blot analysis of lysates from Mouse spleen usingCD79b Rabbit mAb (A25358) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Confocal imaging of A20 cells usingCD79b Rabbit mAb (A25358, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging ofparaffin-embedded Mouse spleen usingCD79b Rabbit mAb (A25358, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
	Confocal imaging ofparaffin-embedded Mouse thymus usingCD79b Rabbit mAb (A25358, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
	Flow cytometry: 1X10 6 C2C12 cells (negative control,left)and A20 (right) cells were surface-stained with Mouse CD79b mAb (A25358,2 µg/mL,orange line) or PE Rabbit IgG isotype control (A24172,5 µl/Test,blue line), followed by PE conjugated Donkey anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry: 1X10 6 A20 cells were surface-stained with PE Rabbit IgG isotype control (A24172,5 µl/Test,left) or Mouse CD79b mAb (A25358,2 µg/mL,right).
	Flow cytometry: 1X10 6 C57BL/6 mouse Splenocytes were surface-stained with Mouse CD79b mAb (A25358,2 µg/mL,orange line) or PE Rabbit IgG isotype control (A24172,5 µl/Test,blue line), followed by PE Donkey anti-rabbit Antibody staining. Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry: 1X10 6 C57BL/6 mouse Splenocytes were surface-stained with PE Rabbit IgG isotype control (A24172,5 µl/Test,left) or Mouse CD79b mAb (A25358,2 µg/mL,right).