[KD Validated] RGAP1 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A24948
- Images (8)
| Article Name: | [KD Validated] RGAP1 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A24948 |
| Supplier Catalog Number: | A24948 |
| Alternative Catalog Number: | ABB-A24948-100UL,ABB-A24948-20UL,ABB-A24948-500UL,ABB-A24948-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | CYK4, CDAN3B, ID-GAP, HsCYK-4, MgcRacGAP, [KD Validated] RGAP1 |
| This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. |
| Application Dilute: | WB,1:2000 - 1:12000|IHC-P,1:100 - 1:400|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,G protein signaling,Small G proteins,Cell Biology Developmental Biology,Cell Cycle,Centrosome,Immunology Inflammation,Cytokines. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Western blot analysis of various lysates, using [KD Validated] RGAP1 Rabbit mAb (A24948) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human testis tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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