Myoglobin Rabbit mAb, Unconjugated, Monoclonal

Article Name:	Myoglobin Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A24206
Supplier Catalog Number:	A24206
Alternative Catalog Number:	ABB-A24206-100UL,ABB-A24206-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	MYOSB, PVALB, Myoglobin

This gene encodes a member of the globin superfamily and is predominantly expressed in skeletal and cardiac muscles. The encoded protein forms a monomeric globular haemoprotein that is primarily responsible for the storage and facilitated transfer of oxygen from the cell membrane to the mitochondria. This protein also plays a role in regulating physiological levels of nitric oxide. Multiple transcript variants encoding distinct isoforms exist for this gene.

Clonality:	Monoclonal
Molecular Weight:	17kDa
NCBI:	4151
UniProt:	P02144
Purity:	Affinity purification
Target:	MB

Application Dilute:	WB,1:5000 - 1:30000\|IF-P,1:200 - 1:2000\|IHC-P,1:2000 - 1:10000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Cardiovascular,Blood,Hypoxia,Heart. Shipping: Ice Bag

	Western blot analysis of various lysates using Myoglobin Rabbit mAb (A24206) at 1:5000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry analysis of paraffin-embeddedHuman skeletal muscle tissue usingMyoglobin Rabbit mAb(A24206) at a dilution of 1:10000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse heart tissue usingMyoglobin Rabbit mAb(A24206) at a dilution of 1:10000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat heart tissue usingMyoglobin Rabbit mAb(A24206) at a dilution of 1:10000 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Confocal imaging of paraffin-embedded Mouse heart tissue using Myoglobin Rabbit mAb (A24206, dilution 1:1600) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Rat heart tissue using Myoglobin Rabbit mAb (A24206, dilution 1:1600) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Human skeletal muscle tissue using Myoglobin Rabbit mAb (A24206, dilution 1:1600) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Mouse skeletal muscle tissue using Myoglobin Rabbit mAb (A24206, dilution 1:1600) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.