HSF1 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	HSF1 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A23895
Supplier Catalog Number:	A23895
Alternative Catalog Number:	ABB-A23895-100UL,ABB-A23895-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, IP, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	HSTF1, HSF1

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.

Clonality:	Monoclonal
Molecular Weight:	57kDa
NCBI:	3297
UniProt:	Q00613
Purity:	Affinity purification
Target:	HSF1

Application Dilute:	WB,1:1000 - 1:4000\|IHC-P,1:200 - 1:2000\|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Signal Transduction,MAPK-JNK Signaling Pathway,Cardiovascular,Heart,Cardiogenesis. Shipping: Ice Bag

	Western blot analysis of lysates from HeLa cells using HSF1 Rabbit mAb (A23895) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of lysates from Rat testis using HSF1 Rabbit mAb (A23895) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HSF1 Rabbit mAb (A23895) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using HSF1 Rabbit mAb (A23895) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using HSF1 Rabbit mAb (A23895) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using HSF1 Rabbit mAb (A23895) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using HSF1 Rabbit mAb (A23895) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunoprecipitation analysis of 300 µg extracts of HeLa cells using 3 µg HSF1 Rabbit mAb (A23895). Western blot was performed from the immunoprecipitate using HSF1 Rabbit mAb (A23895) at a dilition of 1:2000.