CYP51A1 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	CYP51A1 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A23370
Supplier Catalog Number:	A23370
Alternative Catalog Number:	ABB-A23370-100UL,ABB-A23370-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	LDM, CP51, CYP51, CYPL1, P450L1, P450-14DM, CYP51A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene.

Clonality:	Monoclonal
Molecular Weight:	57kDa
NCBI:	1595
UniProt:	Q16850
Purity:	Affinity purification
Target:	CYP51A1

Application Dilute:	WB,1:1000 - 1:5000\|IHC-P,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Endocrine Metabolism,Mitochondrial metabolism,Lipid Metabolism,Cholesterol Metabolism,Lipases,Drug metabolism,Cardiovascular,Lipids. Shipping: Ice Bag

	Western blot analysis of various lysates, using CYP51A1 Rabbit mAb (A23370) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry analysis of paraffin-embeddedHuman lung tissue usingCYP51A1 Rabbit mAb(A23370) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse testis tissue usingCYP51A1 Rabbit mAb(A23370) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman liver cancer tissue usingCYP51A1 Rabbit mAb(A23370) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat liver tissue usingCYP51A1 Rabbit mAb(A23370) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse liver tissue usingCYP51A1 Rabbit mAb(A23370) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat testis tissue usingCYP51A1 Rabbit mAb(A23370) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse kidney tissue usingCYP51A1 Rabbit mAb(A23370) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.