GSK3beta Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A2081
- Images (8)
| Article Name: | GSK3beta Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A2081 |
| Supplier Catalog Number: | A2081 |
| Alternative Catalog Number: | ABB-A2081-1000UL,ABB-A2081-20UL,ABB-A2081-500UL,ABB-A2081-100UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, IP, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | GSK3B, gsk-3beta, GSK3beta |
| The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. |
| Application Dilute: | WB,1:500 - 1:2000|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Cancer,Signal Transduction,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cell Cycle,Centrosome,Cell Cycle Control-G1 S Checkpoint,Cell Adhesion,Microtubules,Wnt -Catenin Signaling Pathway,ESC Pluripotency and Differentiation,Endocrine Metabolism,Carbohydrate metabolism,Insulin Receptor Signaling Pathway,Endocrine and metabolic diseases,Diabetes,Immunology Inflammation,B Cell Receptor Signaling Pathway,NF-kB Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Neurodegenerative Diseases Markers,Other Neurological disorders,Stem Cells,Cardiovascular,Heart,Hypertrophy. Shipping: Ice Bag |
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Western blot analysis of lysates from mouse brain, using GSK3beta Rabbit pAb (A2081) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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Western blot analysis of various lysates using GSK3beta Rabbit pAb (A2081) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
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Western blot analysis of lysates from 293T cells, using GSK3beta Rabbit pAb (A2081) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using GSK3beta Rabbit pAb (A2081) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GSK3beta Rabbit pAb (A2081) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse spleen using GSK3beta Rabbit pAb (A2081) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunofluorescence analysis of C6 cells using GSK3beta Rabbit pAb (A2081) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200 µg extracts of HeLa cells using 3 µg GSK3beta antibody (A2081). Western blot was performed from the immunoprecipitate using GSK3beta antibody (A2081) at a dilution of 1:1000. |
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