MT-ND3 Rabbit pAb, Polyclonal
Catalog Number:
ABB-A17969
- Images (7)
| Article Name: | MT-ND3 Rabbit pAb, Polyclonal |
| Biozol Catalog Number: | ABB-A17969 |
| Supplier Catalog Number: | A17969 |
| Alternative Catalog Number: | ABB-A17969-20UL,ABB-A17969-100UL,ABB-A17969-1000UL,ABB-A17969-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, IP, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Alternative Names: | MTND3, ND3, MT-ND3 |
| Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone. Part of mitochondrial respiratory chain complex I. Implicated in Leber hereditary optic neuropathy, Leigh disease, and Parkinsons disease. |
| Application Dilute: | WB,1:100 - 1:500|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Endocrine Metabolism,Mitochondrial metabolism,Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MT-ND3 Rabbit pAb (A17969) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human appendix using MT-ND3 Rabbit pAb (A17969) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Western blot analysis of various lysates using MT-ND3 Rabbit pAb (A17969) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MT-ND3 Rabbit pAb (A17969) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunofluorescence analysis of L929 cells using MT-ND3 Rabbit pAb (A17969) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of HepG2 cells using MT-ND3 Rabbit pAb (A17969) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200 µg extracts of HeLa cells using 3 µg MT-ND3 antibody (A17969). Western blot was performed from the immunoprecipitate using MT-ND3 antibody (A17969) at a dilution of 1:500. |
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