KIF5B Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A15284
Article Name: KIF5B Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A15284
Supplier Catalog Number: A15284
Alternative Catalog Number: ABB-A15284-100UL,ABB-A15284-20UL,ABB-A15284-1000UL,ABB-A15284-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC-P, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: KNS, KINH, KNS1, UKHC, HEL-S-61, KIF5B
Enables identical protein binding activity, microtubule binding activity, and microtubule motor activity. Involved in several processes, including lysosome localization, natural killer cell mediated cytotoxicity, and positive regulation of protein localization to plasma membrane. Located in centriolar satellite, cytosol, and vesicle.
Clonality: Polyclonal
Molecular Weight: 110kDa
NCBI: 3799
UniProt: P33176
Purity: Affinity purification
Sequence: LQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTV
Target: KIF5B
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:1000|IHC-P,1:50 - 1:100|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Motor Proteins. Shipping: Ice Bag
Immunohistochemistry analysis of paraffin-embedded Mouse lung using KIF5B Rabbit pAb (A15284) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using KIF5B Rabbit pAb (A15284) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Western blot analysis of various lysates using KIF5B Rabbit pAb (A15284) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using KIF5B Rabbit pAb (A15284) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using KIF5B Rabbit pAb (A15284) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of C6 cells using KIF5B Rabbit pAb (A15284) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using KIF5B Rabbit pAb (A15284) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-251 MG cells using KIF5B Rabbit pAb (A15284) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.