GFAP Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A14673
- Images (7)
| Article Name: | GFAP Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A14673 |
| Supplier Catalog Number: | A14673 |
| Alternative Catalog Number: | ABB-A14673-100UL,ABB-A14673-20UL,ABB-A14673-500UL,ABB-A14673-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | ALXDRD, GFAP |
| This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Application Dilute: | WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200|IF-P,1:50 - 1:200|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cell Adhesion,Cytoskeleton,Intermediate Filaments,Neuroscience, Cell Type Marker,Stem Cells,Neural Stem Cells,Astrocyte marker. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Rat brain using GFAP Rabbit pAb (A14673) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat cerebellum using GFAP Rabbit pAb (A14673) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Western blot analysis of various lysates using GFAP Rabbit pAb (A14673) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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Immunohistochemistry analysis of paraffin-embedded Mouse brain using GFAP Rabbit pAb (A14673) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunofluorescence analysis of paraffin-embedded mouse brain using GFAP Rabbit pAb (A14673) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |
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Immunofluorescence analysis of paraffin-embedded rat brain using GFAP Rabbit pAb (A14673) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |
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Immunofluorescence analysis of U-251MG cells using GFAP Rabbit pAb (A14673) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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