CD146/MCAM Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A13927
- Images (7)
| Article Name: | CD146/MCAM Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A13927 |
| Supplier Catalog Number: | A13927 |
| Alternative Catalog Number: | ABB-A13927-100UL,ABB-A13927-20UL,ABB-A13927-500UL,ABB-A13927-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | CD146, MUC18, HEMCAM, METCAM, MelCAM, CD146/MCAM |
| Involved in glomerular filtration and vascular wound healing. Acts upstream of or within angiogenesis. Located in external side of plasma membrane. Biomarker of chronic obstructive pulmonary disease and uveal melanoma. |
| Clonality: | Polyclonal |
| Molecular Weight: | 72kDa |
| NCBI: | 4162 |
| UniProt: | P43121 |
| Purity: | Affinity purification |
| Sequence: | GEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPP |
| Target: | MCAM |
| Antibody Type: | Primary Antibody |
| Application Dilute: | WB,1:100 - 1:500|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Invasion and Metastasis,Signal Transduction,Cell Biology Developmental Biology,Cell Adhesion,Cytoskeleton,Immunology Inflammation,CDs,Stem Cells. Shipping: Ice Bag |
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Western blot analysis of various lysates using CD146/MCAM Rabbit pAb (A13927) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CD146/MCAM Rabbit pAb (A13927) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Western blot analysis of various lysates using CD146/MCAM Rabbit pAb (A13927) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CD146/MCAM Rabbit pAb (A13927) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human lung using CD146/MCAM Rabbit pAb (A13927) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunofluorescence analysis of A375 cells using CD146/MCAM Rabbit pAb (A13927) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of HeLa cells using CD146/MCAM Rabbit pAb (A13927) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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