Neutrophil Elastase (ELANE) Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A13015
- Images (7)
| Article Name: | Neutrophil Elastase (ELANE) Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A13015 |
| Supplier Catalog Number: | A13015 |
| Alternative Catalog Number: | ABB-A13015-100UL,ABB-A13015-20UL,ABB-A13015-1000UL,ABB-A13015-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | GE, NE, HLE, HNE, ELA2, SCN1, PMN-E, Neutrophil Elastase (ELANE) |
| Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. |
| Clonality: | Polyclonal |
| Molecular Weight: | 29 kDa |
| NCBI: | 1991 |
| UniProt: | P08246 |
| Purity: | Affinity purification |
| Sequence: | IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH |
| Target: | ELANE |
| Antibody Type: | Primary Antibody |
| Application Dilute: | WB,1:500 - 1:5000|IF/ICC,1:50 - 1:200|IF-P,1:50 - 1:200|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Extracellular Matrix. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human esophagus using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Western blot analysis of lysates from THP-1 cells, using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunofluorescence analysis of paraffin-embedded mouse bone marrow using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of paraffin-embedded rat bone marrow using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of THP-1 cells using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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