TRIM25 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A12938
- Images (7)
| Article Name: | TRIM25 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A12938 |
| Supplier Catalog Number: | A12938 |
| Alternative Catalog Number: | ABB-A12938-100UL,ABB-A12938-20UL,ABB-A12938-500UL,ABB-A12938-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, IP, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | EFP, Z147, RNF147, ZNF147, TRIM25 |
| The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity. |
| Clonality: | Polyclonal |
| Molecular Weight: | 71kDa |
| NCBI: | 7706 |
| UniProt: | Q14258 |
| Purity: | Affinity purification |
| Sequence: | SLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRP |
| Target: | TRIM25 |
| Antibody Type: | Primary Antibody |
| Application Dilute: | WB,1:500 - 1:2000|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Signal Transduction,Cell Biology Developmental Biology,Growth factors,Ubiquitin. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Human kidney using TRIM25 Rabbit pAb (A12938) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse intestin using TRIM25 Rabbit pAb (A12938) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Western blot analysis of various lysates, using TRIM25 Rabbit pAb (A12938) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
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Immunohistochemistry analysis of paraffin-embedded Rat kidney using TRIM25 Rabbit pAb (A12938) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunofluorescence analysis of A-431 cells using TRIM25 Rabbit pAb (A12938) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of HeLa cells using TRIM25 Rabbit pAb (A12938) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200 µg extracts of HepG2 cells, using 3 µg TRIM25 antibody (A12938). Western blot was performed from the immunoprecipitate using TRIM25 antibody (A12938) at a dilution of 1:1000. |
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