Ku80 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A12338
- Images (7)
| Article Name: | Ku80 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A12338 |
| Supplier Catalog Number: | A12338 |
| Alternative Catalog Number: | ABB-A12338-100UL,ABB-A12338-20UL,ABB-A12338-500UL,ABB-A12338-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, IP, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | KU80, KUB2, Ku86, NFIV, KARP1, KARP-1, Ku80 |
| The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. |
| Application Dilute: | WB,1:1000 - 1:6000|IHC-P,1:200 - 1:800|IF/ICC,1:100 - 1:1000|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,RNA Binding. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Human cervix tissue using Ku80 Rabbit mAb (A12338) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Ku80 Rabbit mAb (A12338) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Western blot analysis of various lysates, using Ku80 Rabbit mAb (A12338) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Ku80 Rabbit mAb (A12338) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using Ku80 Rabbit mAb (A12338) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Confocal imaging of HeLa cells using Ku80 Rabbit mAb (A12338,dilution 1:100)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x. |
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Immunoprecipitation analysis of 300 µg extracts of HeLa cells using 3 µg Ku80 antibody (A12338). Western blot was performed from the immunoprecipitate using Ku80 antibody (A12338) at a dilution of 1:1000. |
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