beta-Tubulin Rabbit mAb, Unconjugated
Catalog Number:
ABB-A12289
- Images (7)
| Article Name: | beta-Tubulin Rabbit mAb, Unconjugated |
| Biozol Catalog Number: | ABB-A12289 |
| Supplier Catalog Number: | A12289 |
| Alternative Catalog Number: | ABB-A12289-200UL,ABB-A12289-100UL,ABB-A12289-500UL,ABB-A12289-1000UL,ABB-A12289-50UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC-P, IP, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | M40, TUBB1, TUBB5, CDCBM6, CSCSC1, OK/SW-cl.56, beta-Tubulin |
| This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. |
| Application Dilute: | WB,1:5000-1:20000|IHC-P,1:50 - 1:200|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cell Cycle,Centrosome,Cytoskeleton,Microtubules. Shipping: Ice Bag |
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Western blot analysis of various lysates using beta-Tubulin Rabbit mAb (A12289) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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Western blot analysis of lysates from 293F cells, using beta-Tubulin Rabbit mAb (A12289) at 1:1000-1:30000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using beta-Tubulin Rabbit mAb (A12289) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunoprecipitation analysis of 300 µg extracts of 293T cells using 3 µg beta-Tubulin Rabbit mAb (A12289). Western blot was performed from the immunoprecipitate using beta-Tubulin Rabbit mAb (A12289) at a dilution of 1:1000. |
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Immunofluorescence analysis of NIH/3T3 cells using beta-Tubulin Rabbit mAb (A12289) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U2OS cells using beta-Tubulin Rabbit mAb (A12289) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 300 µg extracts of 293T cells using 3 µg beta-Tubulin antibody (A12289). Western blot was performed from the immunoprecipitate using beta-Tubulin antibody (A12289) at a dilution of 1:1000. |
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