SQSTM1/p62 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A11483
- Images (7)
| Article Name: | SQSTM1/p62 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A11483 |
| Supplier Catalog Number: | A11483 |
| Alternative Catalog Number: | ABB-A11483-20UL,ABB-A11483-100UL,ABB-A11483-500UL,ABB-A11483-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IP, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | p60, p62, A170, DMRV, OSIL, PDB3, ZIP3, p62B, NADGP, FTDALS3, SQSTM1/p62 |
| This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. |
| Clonality: | Polyclonal |
| Molecular Weight: | 48kDa |
| NCBI: | 8878 |
| UniProt: | Q13501 |
| Purity: | Affinity purification |
| Sequence: | MAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWT |
| Target: | SQSTM1 |
| Antibody Type: | Primary Antibody |
| Application Dilute: | WB,1:500 - 1:1000|IF/ICC,1:50 - 1:100|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Autophagy,Endocrine Metabolism,Mitochondrial metabolism,Cardiovascular,Heart. Shipping: Ice Bag |
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Immunofluorescence analysis of U2OS cells using SQSTM1/p62 Rabbit pAb (A11483) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U2OS cells using SQSTM1/p62 Rabbit pAb (A11483) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Western blot analysis of various lysates using SQSTM1/p62 Rabbit pAb (A11483) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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Immunoprecipitation analysis of 100 µg extracts of HepG2 cells using 3 µg SQSTM1/p62 antibody (A11483). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A11483) at a dilution of 1:1000. |
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Immunofluorescence analysis of U2OS cells using SQSTM1/p62 Rabbit pAb (A11483) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U2OS cells using SQSTM1/p62 Rabbit pAb (A11483) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 100 µg extracts of HepG2 cells using 3 µg SQSTM1/p62 antibody (A11483). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A11483) at a dilution of 1:1000. |
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