Histone H2AX Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A11361
- Images (7)
| Article Name: | Histone H2AX Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A11361 |
| Supplier Catalog Number: | A11361 |
| Alternative Catalog Number: | ABB-A11361-1000UL,ABB-A11361-500UL,ABB-A11361-20UL,ABB-A11361-100UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | H2A.X, H2A/X, H2AFX, Histone H2AX |
| Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. |
| Application Dilute: | WB,1:500 - 1:5000|IHC-P,1:100 - 1:200|IF/ICC,1:50 - 1:400|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat,Other (Wide Range Predicted). ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,Protein phosphorylation,ATM Signaling Pathway. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Human stomach using Histone H2AX Rabbit pAb (A11361) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse brain using Histone H2AX Rabbit pAb (A11361) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Western blot analysis of various lysates using Histone H2AX Rabbit pAb (A11361) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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Immunohistochemistry analysis of paraffin-embedded Rat brain using Histone H2AX Rabbit pAb (A11361) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Confocal immunofluorescence analysis of HeLa cells using Histone H2AX Rabbit pAb (A11361) at dilution of 1:400. Blue: DAPI for nuclear staining. |
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Confocal immunofluorescence analysis of U-2 OS cells using Histone H2AX Rabbit pAb (A11361) at dilution of 1:400. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U2OS cells using Histone H2AX Rabbit pAb (A11361) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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