HLA-DQA1 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	HLA-DQA1 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A11252
Supplier Catalog Number:	A11252
Alternative Catalog Number:	ABB-A11252-100UL,ABB-A11252-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	DQA1, DQ-A1, CELIAC1, HLA-DQA, HLA-DQB1, HLA-DQA1*, HLA-DQA1

HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.

Clonality:	Monoclonal
Molecular Weight:	28kDa
NCBI:	3117
UniProt:	P01909
Purity:	Affinity purification
Target:	HLA-DQA1

Application Dilute:	WB,1:1000 - 1:6000\|IHC-P,1:200 - 1:2000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. Shipping: Ice Bag

	Western blot analysis of various lysates using HLA-DQA1 Rabbit mAb (A11252) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human liver tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.