NRF2 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A11159
- Images (8)
| Article Name: | NRF2 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A11159 |
| Supplier Catalog Number: | A11159 |
| Alternative Catalog Number: | ABB-A11159-100UL,ABB-A11159-20UL,ABB-A11159-500UL,ABB-A11159-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | NRF2, HEBP1, Nrf-2, IMDDHH |
| This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters, many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. |
| Clonality: | Polyclonal |
| Molecular Weight: | 68kDa |
| NCBI: | 4780 |
| UniProt: | Q16236 |
| Purity: | Affinity purification |
| Sequence: | YGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN |
| Target: | NFE2L2 |
| Application Dilute: | WB,1:500 - 1:1000|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Cell Biology Developmental Biology,Endocrine Metabolism,Mitochondrial metabolism,Nucleotide metabolism,Cardiovascular,Heart. Shipping: Ice Bag |
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Western blot analysis of lysates from NIH/3T3 cells using NRF2 Rabbit pAb (A11159) at 1:1000 dilutionincubated overnight at 4°C. NIH/3T3 cells were treated with MG132(10 µM) at 37°C for 90 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
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Western blot analysis of lysates from C6 cells using NRF2 Rabbit pAb (A11159) at 1:1000 dilutionincubated overnight at 4°C. C6 cells were treated with MG132(10 µM) at 37°C for 90 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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Immunohistochemistry analysis of paraffin-embedded Human liver cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunofluorescence analysis of HeLa cells using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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