alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A1011
- Images (7)
| Article Name: | alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A1011 |
| Supplier Catalog Number: | A1011 |
| Alternative Catalog Number: | ABB-A1011-20UL,ABB-A1011-100UL,ABB-A1011-500UL,ABB-A1011-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | ACTSA, alpha-Smooth Muscle Actin (ACTA2) |
| This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. |
| Application Dilute: | WB,1:500 - 1:1000|IF-P,1:50 - 1:200|IHC-P,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat,Cynomolgus monkey. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Microfilaments,Actins,Death Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases Markers,Other Neurological disorders,Stem Cells,Mesenchymal Stem Cells,Cardiovascular,Heart,Contractility. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embeddedHuman tonsil tissue usingalpha-Smooth Muscle Actin (ACTA2) Rabbit pAb(A1011) at a dilution of1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman esophagus tissue usingalpha-Smooth Muscle Actin (ACTA2) Rabbit pAb(A1011) at a dilution of1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Western blot analysis of lysates from Mouse heart, using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A1011) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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Immunohistochemistry analysis of paraffin-embeddedHuman lung tissue usingalpha-Smooth Muscle Actin (ACTA2) Rabbit pAb(A1011) at a dilution of1:1000 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Immunofluorescence analysis of paraffin-embedded rat rectum using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A1011) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of paraffin-embedded human smooth muscle using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A1011) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of paraffin-embedded mouse colon using alpha-Smooth Muscle Actin (ACTA2) Rabbit pAb (A1011) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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