HES1 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A0925
- Images (7)
| Article Name: | HES1 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A0925 |
| Supplier Catalog Number: | A0925 |
| Alternative Catalog Number: | ABB-A0925-100UL,ABB-A0925-20UL,ABB-A0925-1000UL,ABB-A0925-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC-P, IP, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | HHL, HRY, HES-1, bHLHb39, HES1 |
| This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. |
| Application Dilute: | WB,1:1000 - 1:2000|IHC-P,1:1000 - 1:4000|IP,0.5µg-4µg antibody for 400µg-600µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Neuroscience. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using HES1 Rabbit mAb (A0925) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HES1 Rabbit mAb (A0925) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Western blot analysis of various lysates using HES1 Rabbit mAb (A0925) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
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Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using HES1 Rabbit mAb (A0925) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using HES1 Rabbit mAb (A0925) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using HES1 Rabbit mAb (A0925) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunoprecipitation analysis of 600 µg extracts of Rat testis cells using 3 µg HES1 antibody (A0925). Western blot was performed from the immunoprecipitate using HES1 antibody (A0925) at a dilution of 1:500. |
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