Ku70 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A0883
- Images (7)
| Article Name: | Ku70 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A0883 |
| Supplier Catalog Number: | A0883 |
| Alternative Catalog Number: | ABB-A0883-100UL,ABB-A0883-20UL,ABB-A0883-1000UL,ABB-A0883-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | ML8, KU70, TLAA, CTC75, CTCBF, G22P1, Ku70 |
| The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. |
| Clonality: | Polyclonal |
| Molecular Weight: | 70kDa |
| NCBI: | 2547 |
| UniProt: | P12956 |
| Purity: | Affinity purification |
| Sequence: | PYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD |
| Target: | XRCC6 |
| Antibody Type: | Primary Antibody |
| Application Dilute: | WB,1:3000 - 1:18000|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,RNA Binding,Cancer,Cell Biology Developmental Biology,Cell Cycle. Shipping: Ice Bag |
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Western blot analysis of lysates from C6 cells using Ku70 Rabbit pAb (A0883) at 1:3000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
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Immunohistochemistry analysis of paraffin-embedded Rat brain using Ku70 Rabbit pAb (A0883) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Western blot analysis of various lysates using Ku70 Rabbit pAb (A0883) at 1:3000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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Immunohistochemistry analysis of paraffin-embedded Human breast using Ku70 Rabbit pAb (A0883) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human leiomyoma of uterus using Ku70 Rabbit pAb (A0883) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse lung using Ku70 Rabbit pAb (A0883) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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Confocal immunofluorescence analysis of U2OS cells using Ku70 Rabbit pAb (A0883) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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