MED4 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	MED4 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A0623
Supplier Catalog Number:	A0623
Alternative Catalog Number:	ABB-A0623-100UL,ABB-A0623-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	ARC36, VDRIP, DRIP36, TRAP36, HSPC126, MED4

This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Clonality:	Monoclonal
Molecular Weight:	30kDa
NCBI:	29079
UniProt:	Q9NPJ6
Purity:	Affinity purification
Target:	MED4

Application Dilute:	WB,1:1000 - 1:6000\|IHC-P,1:200 - 1:2000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling. Shipping: Ice Bag

	Western blot analysis of various lysates using MED4 Rabbit mAb (A0623) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry analysis of paraffin-embeddedHuman breast cancer tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman breast tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman esophagus tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman colon tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.