Purified, E.coli-derived recombinant human NCOR1. (aa1770-1947, Accession O75376).
NCOR1 (Nuclear receptor Co-Repressor 1) is a 270kD member of the NCoR family of molecules. It is widely expressed, being found in hepatocytes, intestinal crypt cells, neural stem cells, plus immature thymocytes and erythrocytes. NCOR1 is a transcriptional repressor. It forms a complex with HDAC3, TAB2 and ZBTB33, and interacts with a ligand-independent THR:RXR heterodimer bound to select gene promoters. Human NCOR1 is 2440aa in length. It possesses one N-terminal repression domain (aa1-312), two DNA-binding SANT domains (aa437-674) and a second repression domain (aa737-1004). Multiple Ser, Thr and Tyr phosphorylation sites exist that regulate complex dissociation. There are multiple potential splice variants. Short poly Lys motifs serve as substitutions for the C-terminal 1900-1910aa. There is also a 16aa insertion after Glu727, coupled to either a Ile substitution for aa1842-1961, or a six aa substitution for aa31-145. Over aa1770-1947, human NCOR1 shares 96% aa identity with mouse NCOR1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot:1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
