A 14 aa peptide sequence from the C-terminus of mature human motilin (1) was coupled to KLH
Transport of nutrients through the digestive system is highly dependent on gastrointestinal (GI) motility. GI motility disorders include gastroesophageal reflux disease, gastroparesis (diabetic and post surgical), irritable bowel syndrome, and constipation. Motilin, a 22-amino acid peptide hormone that is secreted by enterochromaffin cells of the small intestine, influences gastric motility by inducing interdigestive (phase III) antrum and duodenal contractions. Most recently, an orphan GPCR related to growth hormone secretagogues receptor (GHS-R) has been isolated and characterized from human stomach as the motilin receptor (MTLR or GPR38, 52% identity with GHS-R). The structurally unrelated macrolide antibiotic erythromycin is known to have Motilin-like side effects, including vomiting, nausea, diarrhea, and abdominal muscular discomfort. MTLR is expressed in enteric neurons of the human duodenum and colon. Interestingly, the macrolide antibiotic erythromycin also binds to MTLR, providing a molecular basis for its effects on the human GI tract. Motilin gene (human chromosome 6p21.2) consists of 5 exons spanning approximately 9 kb of genomic DNA. Exon 1 encodes the 5-prime untranslated portion of the motilin mRNA. Exons 2 and 3 encode the signal peptide and the 22-amino acid motilin peptide. The 22-aa mature motilin peptide is highly conserved across species. The motilin prohomrone consists of 115 amino acids and includes a 25-residue N-terminal signal peptide followed by the 22-amino-acid motilin sequence and a long, 68-residue Motilin-related C-terminal peptide. Proteolytic processing of promotilin to motilin occurs at a very unusual and rare Lys-Lys sequence. High-affinity binding sites for motilin have been detected in the GI tract of humans and other species and in the central nervous system of rabbits. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. Full length motilin is ~3.0kD. ELISA: ~1:100,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
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