MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)
Artikelnummer:
USB-M2352-01C
Hersteller Artikelnummer:
M2352-01C
Alternativnummer:
USB-M2352-01C-10
Hersteller:
US Biological
Kategorie:
Sonstiges
Applikation:
WB
P38 MAP kinase (MAPK), also called RK (3) or CSBP (2), is the mammalian homologue of the yeast HOG kinase and participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, alpha, beta, gamma and delta, have been identified so far. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8). Nonphosphorylated p38 MAP Kinase Control Cell. Western Blots: As controls, we recommend using 15ul of phosphorylated and nonphosphorylated p38 MAP Kinase Control Cell Extracts. Boil for 2 minutes prior to use. Nonphosphorylated p38 MAP Kinase Control Cell Extracts: Total cell extracts from C-6 glioma cells prepared without anisomycin treatment serve as a negative control. Phosphorylated p38 MAP Kinase Control Cell Extracts: Total cell extracts from C-6 glioma cells prepared with anisomycin treatment serve as a positive control. Storage and Stability: Long-term storage is recommended at -20C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Reinheit:
Lysate
Formulierung:
Supplied as a liquid in 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 50mM DTT, 0.01% bromophenol blue.
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